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Blog Post Week 10



My previous hypothesis was incorrect. The Freeze and Thaw technique did not produce the best or even the most DNA. In both instances, the Boiling Protocol produce the best quality of bacterial DNA when compared to the other techniques. The TE Boiling, however, produce the most bacterial DNA. This research was essential in the field of microbiology. It confirms that when bacteria come face to face with harsh conditions such as heat, the bacteria will leave behind DNA fragments. In the case of pathology, bactericide can kill off a microbe but if the DNA fragments are left behind then another strain of bacteria can easily incorporate the foreign DNA. This act, called Transformation, is the primary cause of new and more dangerous strains of bacteria. Bacteria must be sterilized to completely eradicate any chances of this. In the field of genetic engineering, the bacterial DNA remains are quite promising for further research. With a proper technique to isolate DNA fragments, further studies can be made to identify the key proteins involved with enabling a bacteria’s virulence and other traits among the bacteria.

Figure 1. Pseudomonas Data 
Figure 2. Corynebacterium Data

1 comment:

  1. Hey you're graphs look very amazing. So when comparing how pure the RNA (DNA) is which method came out to be the most pure. Also you're graphs are very great and labeled, I especially liked the graphs that had color in them makes you're paper stand out and not just another black and white paper.

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