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Blog Post Week 4


I was able to identify my bacteria as Pseudomonas Aeruginosa. I spent all of Monday morning just extracting DNA samples from my bacteria. The first extraction method I use was the TE Freeze and Thaw method. It is a simple procedure. A person must take 1mL of his or her bacteria and centrifuge it for a certain amount of time. Later boil the bacteria, then place it in ice water, boil again, and repeat the freezing and thawing cycle a few times, The goal is to broken down the components of the bacteria so it can lose DNA particles. Some tests are more effective than others, of course. Well, I got to keep this up because all those DNA are not going to extract themselves. Ciao.

2 comments:

  1. Hi Barierane, were you able to successfully extract DNA using the methods you tested?

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    1. Yes! The only issue is that some of the tests such as the Boiling method produce a low quantity and quality of DNA so IO wasn't so sure if the test was effective.

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