Blog Post Week 7

Hi, everybody! Today I finished extracting my DNA samples from my two bacteria and began my Agarose Gel Electrophoresis Protocol. This is a long step process that requires gloves because the SYBR green binds to the nucleic acids that on is on the human skin. The first few  steps is heating and mixing agarose, TAE buffer, and water to a right consistency before making a gel to examine the DNA. My first DNA, Pseudomonas Aeruginosa, produce an excellent result with DNA streaks running down the gel. My second DNA, Corynebacterium, produce a small amount of DNA example with DNA running in the opposite direction. We speculate that my first DNA was a gram negative so it function in a position that I would expect it to in the test while my second DNA was a gram positive so it function in a different direction. I will redo the test to make sure it is right.

An image of my Corynebacterium Agarose Gel Electrophoresis.